Agarose Droplet Microfluidics for Highly Parallel and Efficient Emulsion Pcr
نویسندگان
چکیده
An agarose droplet method was developed for highly parallel and efficient single copy emulsion PCR. By capitalizing on the unique thermal-sensitive sol-gel switching property of agarose, PCR is efficiently performed in agarose solution droplets, which is gelated to agarose beads after PCR to capture amplicons to maintain the monoclonality of each droplet. This method allows high throughput generation of uniform droplets and enables high PCR efficiency, making it a promising platform for many single copy genetic studies.
منابع مشابه
Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR.
Agarose emulsion droplet microfluidic technology for single copy emulsion PCR (ePCR) is a suitable technique for the detection of single copy DNA molecules. It improves the traditional ePCR by employing agarose with low melting and low gelling temperatures, which is coupled with PCR forward primers using Schiff-base reaction. Highly uniform monodisperse nanoliter agarose droplets each containin...
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